A modification by Bevis of the original CLED medium of Mackey & Sandys. This formulation uses a double indicator system (Andrade’s (acid fuchsin) and bromothymol blue) to improve differentiation of lactose and non-lactose fermenting organisms. The lack of sodium chloride also prevents the swarming of Proteus spp.
- Storage temperature: 2 to 8°C
- Appearance: firm gel
- Colour: green/blue
- Recommended incubation: aerobically at 37°C for 16 to 24 hours
- pH: 7.5 ± 0.2
- Plate size: 90mm
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